Details

HSA cell-tracking & analysis in real-time

HSA deep learning


Tracking of cellular morphology changes and behavior in concert with surrounding cells. Cells with increasing area are marked blue, decreasing area red. Length of individual cell borders, circumference, area and percentile change, compared to previous frame (Δ area, Δ circum.), are tracked in real time.

HSA Deep Learning:
  • + Cell border gaps in the original image are closed intelligently
  • + Automatic tracking of length of individual cell borders, cellular morphology changes and behavior in concert with surrounding cells
  • + Visual representation of selected morphology changes (Area, circularity, neighbor cell behavior etc.)
  • + Simple export of all statistical data into an Excel-file

Standard cell-tracking

Standard U-Net Deep Learning


Simple intensity dependent annotation of cell borders vs original image





Standard U-Net deep learning:
  • – Gaps in the original image are not closed, therefore subsequent statistical analysis is impaired
  • – Tracking of cellular parameters difficult or only possible by additional programs
  • – No visual representation of selected changes in cellular morphology



Counting and segmentation of
cells in chamber slides

Detection and segmentation of nuclei and cytoplasm in cultured cells in chamber slides.




Segmentation of Tumor
and Stroma cells

Segmentation of nuclei and cytoplasm in tumour cells and cytoplasmic regions in stroma cells. If nuclear staining is not present or unspecific, nuclei can be detected by nuclear “holes” in cytoplasmic staining.



Kidney Tool

Segmentation of glomeruli and tubuli by using a Deep Learning model for brightfield data.



Kidney Tool

Segmentation of glomeruli and their compartments by using a tensorflow model for brightfield data.



Cell Counting

Segmentation and counting of cells.  






Endoplasmic Reticulum

The endoplasmic reticulum (ER) is a dynamic structure consisting of branched domains and tubular sections. Automatic segmentation opens up new possibilities for quantitative analysis and efficient representation of this membrane structure at the ultra-structural level.



Scratch Assay

Analysis of samples with scratches and the change of cell area over time.